ABSTRACT
Objective To investigate the expression of Ki-67and NF-κB in cervical lesion and their correlation with HPV infec tion.Methods 70 cases with surgically resected or cervical biopsy (70 cases cervical specimens) were selected as the research object from January 2015 to January 2017 in our hospital.The expression of Ki-67,NF-κB and PCNA were detected by immune cell chemical dyeing method,and making the correlation analysis by Spearman.Results The expression rate of Ki-67,NF-κB and PCNA in CIN and cervical invasive squamous carcinoma tissues were significantly higher than that of chronic cervicitis tissues.Spearman rank correlation analysis showed,there was a positively correlation of Ki-67 and PCNA(r=0.582,P＜0.05),there was a positively correlation of NF-κB and PCNA(r=0.603,P＜0.05),there was a positively correlation of Ki-67 and HPV infection (r=0.601,P＜ 0.05),there was a positively correlation of NF-κB and HPV infection (r=0.596,P＜0.05),here was a positively correlation of PCNA expression and HPV infection(r=0.647,P＜0.05).Conclusion There is a difference of positive expression rate of Ki-67,NFκB in different cervical lesions organizations,and Ki-67,NF-κB are associated with HPV infection,they might be involved in cervical lesions in the process,could be used as indexes for identification of cervical disease,diagnosis and evaluation.
ABSTRACT
Objective To investigate the expression of Ki-67and NF-κB in cervical lesion and their correlation with HPV infec tion.Methods 70 cases with surgically resected or cervical biopsy (70 cases cervical specimens) were selected as the research object from January 2015 to January 2017 in our hospital.The expression of Ki-67,NF-κB and PCNA were detected by immune cell chemical dyeing method,and making the correlation analysis by Spearman.Results The expression rate of Ki-67,NF-κB and PCNA in CIN and cervical invasive squamous carcinoma tissues were significantly higher than that of chronic cervicitis tissues.Spearman rank correlation analysis showed,there was a positively correlation of Ki-67 and PCNA(r=0.582,P＜0.05),there was a positively correlation of NF-κB and PCNA(r=0.603,P＜0.05),there was a positively correlation of Ki-67 and HPV infection (r=0.601,P＜ 0.05),there was a positively correlation of NF-κB and HPV infection (r=0.596,P＜0.05),here was a positively correlation of PCNA expression and HPV infection(r=0.647,P＜0.05).Conclusion There is a difference of positive expression rate of Ki-67,NFκB in different cervical lesions organizations,and Ki-67,NF-κB are associated with HPV infection,they might be involved in cervical lesions in the process,could be used as indexes for identification of cervical disease,diagnosis and evaluation.
ABSTRACT
Herb-glycosides are main active elements of Zhongcaoyao (Chinese traditional medicines, Chinese medical herbs). However, the herb-glycoside structures are not optimal active structure for the human bodies. After orally taken up, the herb-glycosides of Zhongcaoyao could be changed into other more active structures by the digestive system such as enzymes and intestinal microorganisms; then degraded and absorbed in the human body and play the real role of pharmic effect; but only a small amount could be changed and controlled by circadian state of the human body. If this biochange of herb-glycosides to more active structures in vivo was finished in vitro, it is very useful for the development of the Chinese traditional medicines, new plant medicines, health food, and function cosmetics. To biotransformate herb-glycosides to more active structure, this paper introduced the studies of author's team on the new microorganism isolation of the special herb-glycosidases and enzyme fermentation, the special enzyme purification and characterization.